Part:BBa_K1149052:Design

Constitutive phaCAB

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 627
  Illegal BglII site found at 1452
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 604
  Illegal NgoMIV site found at 916
  Illegal NgoMIV site found at 1195
  Illegal NgoMIV site found at 1847
  Illegal NgoMIV site found at 1869
  Illegal NgoMIV site found at 2491
  Illegal NgoMIV site found at 2632
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 3713
  

Design Notes

Native promoter and RBS changed to strong constitutive promoter J23104 + B0034

Partial sequence of the constitutive phaCAB construct (BBa_K1149052):